Plant Diversity ›› 2009, Vol. 31 ›› Issue (05): 415-420.DOI: 10.3724 SP.J.1143.2009.09175
• Articles • Previous Articles Next Articles
LEI Qi-Yi , ZHOU Jiang-Ju , ZHANG Wen-Hua
Received:
Online:
Published:
Contact:
雷启义, 周江菊, 张文华
通讯作者:
Abstract: The interacting of MinD and MinC and MinE proteins of Escherichia coli are required for proper placement of the division septum at midcell, The MinD protein plays a key role in the course of bacterial cell and plant chloroplast division. The over production of MinD protein can block the normal cell division and lead to a filamentation cell . In plant cells, There are clear similarities between bacterial and plastid division, but limited information exists regarding the mechanism of plastid division in higher plants . Here we constructed a expression plasmid encoding the full-length CrMinD, anChlamydomonas reinhardtii homologue of the bacterial MinD . In agreement with cell division studies in bacteria over expression of CrMinD in E. coli results in filamentation cell formation . The sesuggestion that CrMinD is an evolutionary conserved cell division protein .Meanwhile , the result lays a certain foundation for studying on the division mechanism of plant chloroplast.
Key words: Chloroplast
摘要： 细胞或质体中部正确分裂位点的选择是MinD 蛋白与其他Min 蛋白(MinC􊄯E ) 相互作用的结果, MinD 蛋白在原核细胞以及植物叶绿体的分裂过程中发挥着重要的作用。细胞中MinD 蛋白浓度的明显升高可影响正常细胞的分裂过程而产生丝状体细胞。为了研究叶绿体分裂蛋白CrMinD 的保守功能, 构建了衣藻CrMinD-gfp 的原核表达重组质粒进行了原核功能验证。试验结果表明, 衣藻CrMinD 蛋白的过量表达严重影响了大肠杆菌的分裂, 其在原核细胞中运动和定位与用GFP 标记的原核细胞MinD 蛋白具有相似性。更进一步证明了叶绿体分裂同源物CrMinD 蛋白与原核细胞MinD 蛋白有着相似的功能, 是一个进化上功能保守的蛋白。同时, 这一结果也为研究植物细胞中质体的分裂机制奠定了一定的基础。
关键词: 叶绿体, MinD 蛋白, 基因表达, 细胞分裂
CLC Number:
Q 71
LEI Qi-Yi , ZHOU Jiang-Ju , ZHANG Wen-Hua. The Conservative Function of Chloroplast Division Associated CrMinD Protein[J]. Plant Diversity, 2009, 31(05): 415-420.
雷启义, 周江菊, 张文华 . 叶绿体分裂相关蛋白CrMinD 的保守功能[J]. Plant Diversity, 2009, 31(05): 415-420.
/ Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.integrativebiology.ac.cn/pd/EN/10.3724 SP.J.1143.2009.09175
https://www.integrativebiology.ac.cn/pd/EN/Y2009/V31/I05/415
